Rajan Singh, PhD
Title Professor
Institution Charles R. Drew University of Medicine and Science
Department Internal Medicine
School College of Medicine
Address 1731 E. 120th Street, Hawkins Building


Telephone (323) 563-5828
Fax
Email
Overview
I have long standing interest and research experience in specific areas of androgen biology and aging research. For last 16 years of my independent career, I have been interested in delineating the molecular mechanism of androgen action on muscle and fat mass. My initial research work led to the identification of follistatin (Fst) as a novel target of androgen action via activation of Wnt/ß-catenin signaling pathway, and subsequent identification of anabolic pathways that are differentially responsive to testosterone in muscle and prostate tissues. Using a systematic and comprehensive genetic approach, we identified a novel role of Fst in controlling brown adipose characteristics. My research laboratory demonstrated that Fst over-expression both in-vitro and in-vivo favorably alters lipidomic/metabolomic profiles implicated in lipoprotein and energy metabolism. This work is the first to demonstrate the action of Fst in targeting both browning of white adipose tissue (WAT) and activation of classical brown adipose tissue (BAT) through distinct molecular mechanisms and have tremendous therapeutic potential of Fst for the treatment of obesity and related metabolic syndromes. These novel information regarding potential beneficial effects of Fst has been awarded US patent (US9682093B2) entitled “Composition and Methods for Treating or Preventing Metabolic Syndrome Disorders”. In collaboration with UCLA investigators, we have recently identified a critical role for Fst-induced browning during its antiatherogenic action. Currently, we are investigating the pro-browning and anti-atherogenic role of Fst in details and identifying key molecular targets involved in mitigating dyslipidemia and the development of atherosclerosis using several gain-of-function (adipose-specific Fst transgenic mouse models generated in our laboratory) and loss-of function using Fst-floxed (Fst fl/fl) mouse models. Over the years, my research has been supported by several NIH grants.
Education & Training
Teaching
NIH Awarded Grants
Publications
1. Singh R, Sarkar A, Guria C, Nicholl RJT, Chakraborty S, Bolotin KI, Ghosh S. Giant Tunable Mechanical Nonlinearity in Graphene-Silicon Nitride Hybrid Resonator. Nano Lett. 2020 May 21.
  View in: PubMed
 
2. Rawat AK, Pal K, Singh R, Anand A, Gupta S, Kishore D, Singh S, Singh RK. The CD200-CD200R cross-talk helps Leishmania donovani to down regulate macrophage and CD4+CD44+ T cells effector functions in an NF?B independent manner. Int J Biol Macromol. 2020 May 15; 151:394-401.
  View in: PubMed
 
3. Rai SN, Zahra W, Singh SS, Birla H, Keswani C, Dilnashin H, Rathore AS, Singh R, Singh RK, Singh SP. Anti-inflammatory Activity of Ursolic Acid in MPTP-Induced Parkinsonian Mouse Model. Neurotox Res. 2019 Oct; 36(3):452-462.
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4. Mallick S, Benson R, Giridhar P, Rajan Singh A, Rath GK. Demography, patterns of care and survival outcomes in patients with malignant tumors of trachea: A systematic review and individual patient data analysis of 733 patients. Lung Cancer. 2019 06; 132:87-93.
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5. Singh AK, Yadav S, Chauhan BS, Nandy N, Singh R, Neogi K, Roy JK, Srikrishna S, Singh RK, Prakash P. Classification of Clinical Isolates of Klebsiella pneumoniae Based on Their in vitro Biofilm Forming Capabilities and Elucidation of the Biofilm Matrix Chemistry With Special Reference to the Protein Content. Front Microbiol. 2019; 10:669.
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6. Jain N, Paroha R, Singh RK, Mishra SK, Chaurasiya SK, Singh RA, Singh J. Synthesis and Rational design of Europium and Lithium Doped Sodium Zinc Molybdate with Red Emission for Optical Imaging. Sci Rep. 2019 02 21; 9(1):2472.
  View in: PubMed
 
7. Singh R, Nicholl RJT, Bolotin KI, Ghosh S. Motion Transduction with Thermo-mechanically Squeezed Graphene Resonator Modes. Nano Lett. 2018 11 14; 18(11):6719-6724.
  View in: PubMed
 
8. Singh R, Pervin S, Lee SJ, Kuo A, Grijalva V, David J, Vergnes L, Reddy ST. Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases. Diabetes Metab Syndr Obes. 2018; 11:65-84.
  View in: PubMed
 
9. Singh R, Ghoshal UC, Kumar S, Mittal B. Genetic variants of immune-related genes IL17F and IL10 are associated with functional dyspepsia: A case-control study. Indian J Gastroenterol. 2017 Sep; 36(5):343-352.
  View in: PubMed
 
10. Pervin S, Singh V, Tucker A, Collazo J, Singh R. Modulation of transforming growth factor-ß/follistatin signaling and white adipose browning: therapeutic implications for obesity related disorders. Horm Mol Biol Clin Investig. 2017 Sep 09; 31(2).
  View in: PubMed
 
11. Guleria A, Karyampudi A, Singh R, Khetrapal CL, Verma A, Ghoshal UC, Kumar D. Mapping of Brain Activations to Rectal Balloon Distension Stimuli in Male Patients with Irritable Bowel Syndrome Using Functional Magnetic Resonance Imaging. J Neurogastroenterol Motil. 2017 Jul 30; 23(3):415-427.
  View in: PubMed
 
12. Singh R, Braga M, Reddy ST, Lee SJ, Parveen M, Grijalva V, Vergnes L, Pervin S. Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues. Endocrinology. 2017 05 01; 158(5):1217-1230.
  View in: PubMed
 
13. Karyampudi A, Ghoshal UC, Singh R, Verma A, Misra A, Saraswat VA. Esophageal Acidification During Nocturnal Acid-breakthrough with Ilaprazole Versus Omeprazole in Gastroesophageal Reflux Disease. J Neurogastroenterol Motil. 2017 Apr 30; 23(2):208-217.
  View in: PubMed
 
14. Agarwal RA, Gupta NK, Singh R, Nigam S, Ateeq B. Ag/AgO Nanoparticles Grown via Time Dependent Double Mechanism in a 2D Layered Ni-PCP and Their Antibacterial Efficacy. Sci Rep. 2017 03 21; 7:44852.
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15. Ghoshal UC, Singh R. Frequency and risk factors of functional gastro-intestinal disorders in a rural Indian population. J Gastroenterol Hepatol. 2017 Feb; 32(2):378-387.
  View in: PubMed
 
16. Singh RK, Kumar P, Mahalingam K. Molecular genetics of human obesity: A comprehensive review. C R Biol. 2017 Feb; 340(2):87-108.
  View in: PubMed
 
17. Singh RK, Mahalingam K. In silico approach to identify non-synonymous SNPs in human obesity related gene, MC3R (melanocortin-3-receptor). Comput Biol Chem. 2017 Apr; 67:122-130.
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18. Shankar A, Roy S, Malik A, Rath GK, Julka PK, Kamal VK, Barnwal K, Upadhyaya S, Singh R, Srivastava V. Level of Awareness of Various Aspects of Lung Cancer Among College Teachers in India: Impact of Cancer Awareness Programmes in Prevention and Early Detection. J Cancer Educ. 2016 12; 31(4):709-714.
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19. Martinez L, Thames E, Kim J, Chaudhuri G, Singh R, Pervin S. Increased sensitivity of African American triple negative breast cancer cells to nitric oxide-induced mitochondria-mediated apoptosis. BMC Cancer. 2016 07 29; 16:559.
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20. Singh R, Mittal B, Ghoshal UC. Functional dyspepsia is associated with GNß3 C825T and CCK-AR T/C polymorphism. Eur J Gastroenterol Hepatol. 2016 Feb; 28(2):226-32.
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21. Singh R, Parveen M, Basgen JM, Fazel S, Meshesha MF, Thames EC, Moore B, Martinez L, Howard CB, Vergnes L, Reue K, Pervin S. Increased Expression of Beige/Brown Adipose Markers from Host and Breast Cancer Cells Influence Xenograft Formation in Mice. Mol Cancer Res. 2016 Jan; 14(1):78-92.
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22. Singh R, Ghoshal UC, Misra A, Mittal B. Achalasia Is Associated With eNOS4a4a, iNOS22GA, and nNOS29TT Genotypes: A Case-control Study. J Neurogastroenterol Motil. 2015 Jul 30; 21(3):380-9.
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23. Grimm S, Jennek S, Singh R, Enkelmann A, Junker K, Rippaus N, Berndt A, Friedrich K. Malignancy of bladder cancer cells is enhanced by tumor-associated fibroblasts through a multifaceted cytokine-chemokine loop. Exp Cell Res. 2015 Jul 01; 335(1):1-11.
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24. Shankar A, Rath G, Roy S, Malik A, Bhandari R, Kishor K, Barnwal K, Upadyaya S, Srivastava V, Singh R. Level of awareness of cervical and breast cancer risk factors and safe practices among college teachers of different states in india: do awareness programmes have an impact on adoption of safe practices? Asian Pac J Cancer Prev. 2015; 16(3):927-32.
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25. Singh R, Braga M, Pervin S. Regulation of brown adipocyte metabolism by myostatin/follistatin signaling. Front Cell Dev Biol. 2014; 2:60.
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26. Joshi RS, Tanpure RS, Singh RK, Gupta VS, Giri AP. Resistance through inhibition: ectopic expression of serine protease inhibitor offers stress tolerance via delayed senescence in yeast cell. Biochem Biophys Res Commun. 2014 Sep 26; 452(3):361-8.
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27. Ghoshal UC, Singh R. Pathogenesis of irritable bowel syndrome: is it really in the gene? J Neurogastroenterol Motil. 2014 Jul 31; 20(3):284-6.
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28. Braga M, Reddy ST, Vergnes L, Pervin S, Grijalva V, Stout D, David J, Li X, Tomasian V, Reid CB, Norris KC, Devaskar SU, Reue K, Singh R. Follistatin promotes adipocyte differentiation, browning, and energy metabolism. J Lipid Res. 2014 Mar; 55(3):375-84.
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29. Jasuja R, Costello JC, Singh R, Gupta V, Spina CS, Toraldo G, Jang H, Li H, Serra C, Guo W, Chauhan P, Narula NS, Guarneri T, Ergun A, Travison TG, Collins JJ, Bhasin S. Combined administration of testosterone plus an ornithine decarboxylase inhibitor as a selective prostate-sparing anabolic therapy. Aging Cell. 2014 Apr; 13(2):303-10.
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30. Singh R, Avliyakulov NK, Braga M, Haykinson MJ, Martinez L, Singh V, Parveen M, Chaudhuri G, Pervin S. Proteomic identification of mitochondrial targets of arginase in human breast cancer. PLoS One. 2013; 8(11):e79242.
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31. Braga M, Pervin S, Norris K, Bhasin S, Singh R. Inhibition of in vitro and in vivo brown fat differentiation program by myostatin. Obesity (Silver Spring). 2013 Jun; 21(6):1180-8.
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32. Srivastava A, Mishra A, Singh R, Rai R, Srivastava N, Mittal B. Multi-analytic approach elucidates significant role of hormonal and hepatocanalicular transporter genetic variants in gallstone disease in North Indian population. PLoS One. 2013; 8(4):e59173.
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33. Pervin S, Hewison M, Braga M, Tran L, Chun R, Karam A, Chaudhuri G, Norris K, Singh R. Down-regulation of vitamin D receptor in mammospheres: implications for vitamin D resistance in breast cancer and potential for combination therapy. PLoS One. 2013; 8(1):e53287.
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34. Kumar S, Ranjan P, Mittal B, Singh R, Ghoshal UC. Lactase persistence/non-persistence genetic variants in irritable bowel syndrome in an endemic area for lactose malabsorption. J Gastroenterol Hepatol. 2012 Dec; 27(12):1825-30.
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35. Ghoshal UC, Singh R. Functional dyspepsia: the Indian scenario. J Assoc Physicians India. 2012 Mar; 60 Suppl:6-8.
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36. Braga M, Bhasin S, Jasuja R, Pervin S, Singh R. Testosterone inhibits transforming growth factor-ß signaling during myogenic differentiation and proliferation of mouse satellite cells: potential role of follistatin in mediating testosterone action. Mol Cell Endocrinol. 2012 Mar 05; 350(1):39-52.
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37. Kumar R, Zakharov MN, Khan SH, Miki R, Jang H, Toraldo G, Singh R, Bhasin S, Jasuja R. The dynamic structure of the estrogen receptor. J Amino Acids. 2011; 2011:812540.
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38. Ghoshal UC, Singh R, Chang FY, Hou X, Wong BC, Kachintorn U. Epidemiology of uninvestigated and functional dyspepsia in Asia: facts and fiction. J Neurogastroenterol Motil. 2011 Jul; 17(3):235-44.
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39. Singh R, Rowan J, Burton C, Galletly C. How effective is a hospital at home service for people with acute mental illness? Australas Psychiatry. 2010 Dec; 18(6):512-6.
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40. Pervin S, Chaudhuri G, Singh R. NO to breast: when, why and why not? Curr Pharm Des. 2010; 16(4):451-62.
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41. Singh R, Bhasin S, Braga M, Artaza JN, Pervin S, Taylor WE, Krishnan V, Sinha SK, Rajavashisth TB, Jasuja R. Regulation of myogenic differentiation by androgens: cross talk between androgen receptor/ beta-catenin and follistatin/transforming growth factor-beta signaling pathways. Endocrinology. 2009 Mar; 150(3):1259-68.
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42. Gupta V, Bhasin S, Guo W, Singh R, Miki R, Chauhan P, Choong K, Tchkonia T, Lebrasseur NK, Flanagan JN, Hamilton JA, Viereck JC, Narula NS, Kirkland JL, Jasuja R. Effects of dihydrotestosterone on differentiation and proliferation of human mesenchymal stem cells and preadipocytes. Mol Cell Endocrinol. 2008 Dec 16; 296(1-2):32-40.
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43. Pervin S, Tran AH, Zekavati S, Fukuto JM, Singh R, Chaudhuri G. Increased susceptibility of breast cancer cells to stress mediated inhibition of protein synthesis. Cancer Res. 2008 Jun 15; 68(12):4862-74.
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44. Pervin S, Singh R, Chaudhuri G. Nitric oxide, N omega-hydroxy-L-arginine and breast cancer. Nitric Oxide. 2008 Sep; 19(2):103-6.
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45. Artaza JN, Singh R, Ferrini MG, Braga M, Tsao J, Gonzalez-Cadavid NF. Myostatin promotes a fibrotic phenotypic switch in multipotent C3H 10T1/2 cells without affecting their differentiation into myofibroblasts. J Endocrinol. 2008 Feb; 196(2):235-49.
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46. Pervin S, Singh R, Hernandez E, Wu G, Chaudhuri G. Nitric oxide in physiologic concentrations targets the translational machinery to increase the proliferation of human breast cancer cells: involvement of mammalian target of rapamycin/eIF4E pathway. Cancer Res. 2007 Jan 01; 67(1):289-99.
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47. Singh R, Artaza JN, Taylor WE, Braga M, Yuan X, Gonzalez-Cadavid NF, Bhasin S. Testosterone inhibits adipogenic differentiation in 3T3-L1 cells: nuclear translocation of androgen receptor complex with beta-catenin and T-cell factor 4 may bypass canonical Wnt signaling to down-regulate adipogenic transcription factors. Endocrinology. 2006 Jan; 147(1):141-54.
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48. Jasuja R, Ramaraj P, Mac RP, Singh AB, Storer TW, Artaza J, Miller A, Singh R, Taylor WE, Lee ML, Davidson T, Sinha-Hikim I, Gonzalez-Cadavid N, Bhasin S. Delta-4-androstene-3,17-dione binds androgen receptor, promotes myogenesis in vitro, and increases serum testosterone levels, fat-free mass, and muscle strength in hypogonadal men. J Clin Endocrinol Metab. 2005 Feb; 90(2):855-63.
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49. Pervin S, Singh R, Freije WA, Chaudhuri G. MKP-1-induced dephosphorylation of extracellular signal-regulated kinase is essential for triggering nitric oxide-induced apoptosis in human breast cancer cell lines: implications in breast cancer. Cancer Res. 2003 Dec 15; 63(24):8853-60.
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50. Bhasin S, Taylor WE, Singh R, Artaza J, Sinha-Hikim I, Jasuja R, Choi H, Gonzalez-Cadavid NF. The mechanisms of androgen effects on body composition: mesenchymal pluripotent cell as the target of androgen action. J Gerontol A Biol Sci Med Sci. 2003 Dec; 58(12):M1103-10.
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51. Pervin S, Singh R, Chaudhuri G. Nitric-oxide-induced Bax integration into the mitochondrial membrane commits MDA-MB-468 cells to apoptosis: essential role of Akt. Cancer Res. 2003 Sep 01; 63(17):5470-9.
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52. Singh R, Artaza JN, Taylor WE, Gonzalez-Cadavid NF, Bhasin S. Androgens stimulate myogenic differentiation and inhibit adipogenesis in C3H 10T1/2 pluripotent cells through an androgen receptor-mediated pathway. Endocrinology. 2003 Nov; 144(11):5081-8.
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53. Singh R, Pervin S, Chaudhuri G. Caspase-8-mediated BID cleavage and release of mitochondrial cytochrome c during Nomega-hydroxy-L-arginine-induced apoptosis in MDA-MB-468 cells. Antagonistic effects of L-ornithine. J Biol Chem. 2002 Oct 04; 277(40):37630-6.
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Funding Opportunities

Click terms below for current NIH/NSF funding opportunities.
  • Follistatin


  • Adipose Tissue, Brown


  • Breast Neoplasms


  • Cell Differentiation


  • Dyspepsia


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Keyword
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Keywords   
Follistatin
Adipose Tissue, Brown
Breast Neoplasms
Cell Differentiation
Dyspepsia
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Co-Authors  
Artaza, Jorge
Basgen, John
Gonzalez-Cadavid, Nestor
Pervin, Shehla
Sinha-Hikim, Indrani
See all (9) people
Physical Neighbors  
Basgen, John
Davidson, Mayer
Gonzalez-Cadavid, Nestor
Friedman, Theodore
Chaban, Victor


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